硫酸右旋糖苷对子宫内膜癌细胞生物学行为的影响

The Effect of Dextran Sulfate on the Biological Behavior of Endometrial Cancer Cells

目的:探讨硫酸右旋糖苷(DS)对子宫内膜腺癌细胞(HEC-1A、Ishikawa)增殖、迁移、侵袭及凋亡的影响及可能的相关分子机制。方法:培养子宫内膜腺癌细胞(HEC-1A、Ishikawa),设立对照组与实验组,分别予以培养基与相应浓度DS处理,采用平板克隆形成实验、CCK8实验检测DS对细胞增殖能力的影响;划痕实验检测DS对细胞迁移能力的影响;Transwell侵袭实验检测DS对细胞侵袭能力的影响;流式细胞术检测DS对细胞凋亡率的影响;Western Blot实验验证DS对细胞凋亡蛋白(Bax、Bcl-2)表达的影响。结果:CCK8实验结果显示,与对照组相比,实验组(0.2%、0.4%、0.6%、0.8% DS)肿瘤细胞增殖明显减少(P 【0.05);克隆形成实验结果显示,与对照组相比,实验组(0.8% DS)肿瘤细胞集落形成数量明显减少(P 【0.05);划痕实验结果显示,与对照组相比,实验组(0.8% DS)划痕愈合率均明显降低(P 【0.05);Transwell侵袭实验结果显示,与对照组相比,实验组(0.8% DS)细胞侵袭能力明显降低(P 【0.05);流式细胞术结果显示,与对照组相比,实验组(0.8% DS)细胞凋亡率明显增加(P 【0.05)。Western Blot实验结果显示,与对照组相比,实验组(0.8% DS)凋亡蛋白Bax表达水平增加,抗凋亡蛋白Bcl-2表达水平明显降低(P 【0.05),Bax/Bcl-2比值明显升高(P 【0.05)。结论:DS能够抑制子宫内膜腺癌细胞(HEC-1A、Ishikawa)的增殖、迁移、侵袭能力,促进细胞凋亡。

Objective: To investigate the effects of dextran sulfate (DS) on the proliferation, migration, invasion and apoptosis of endometrial cancer cells (HEC-1A, Ishikawa) and the possible related molecular mechanisms. Methods: Human endometrial cancer cells (HEC-1A, Ishikawa) were cultured, the control groups were treated with culture medium, and the experimental groups were treated with DS in corresponding concentration, respectively. The effect of DS on cell proliferation was examined by plate clone formation assay and CCK8 assay;the effect of DS on cell migration ability was examined by scratch assay;the effect of DS on cell invasion ability was examined by Transwell invasion assay;the effect of DS on apoptosis rate was examined by flow cytometry;the effect of DS on apoptosis protein (Bax, Bcl-2) expression was verified by Western Blot. Results: The results of CCK8 cell proliferation assay showed that tumor cell proliferation was significantly reduced in the experimental group (0.2%, 0.4%, 0.6%, 0.8% DS) compared with the control group(P <0.05);the results of clone formation assay showed that tumor cell colony formation was significantly reduced in the experimental group (0.8% DS) compared with the control group (P <0.05);the results of the scratch assay showed that the scratch healing rate was significantly lower in the experimental group (0.8% DS) compared with the control group (P <0.05);the results of the Transwell invasion assay showed that the cell invasion ability was significantly lower in the experimental group (0.8% DS) compared with the control group (P <0.05);the results of flow cytometry showed that the cell apoptosis rate was significantly higher in the experimental group (0.8% DS) compared with the control group (P <0.05);the results of Western Blot showed that the expression level of apoptotic protein Bax increased, the expression level of anti-apoptotic protein Bcl-2 decreased significantly (P <0.05) and the Bax/Bcl-2 ratio increased significantly in the experimental group (0.8% DS) compared with the control group (P <0.05). Conclusion: DS can inhibit human endometrial cancer cells (HEC-1A, Ishikawa) proliferation, migration and invasion, and promote apoptosis.