RNA m5C甲基化修饰及其检测技术在肿瘤中的研究进展

Research Progress of RNA m5C Methylation Modification and Its Detection Technology in Tumors

RNA甲基化修饰作为转录后修饰的重要组成部分,主要包括N6-甲基腺苷(m6A)、5-甲基胞嘧啶(m5C)、N1-甲基腺苷(m1A)三种修饰类型。以RNA亚硫酸氢盐测序(RNA-BisSeq)为代表的单碱基分辨率检测技术的发展推动了RNA m5C甲基化修饰的研究。由甲基转移酶、去甲基化酶以及甲基化识别蛋白三个主要成分构成的RNA m5C甲基化修饰调控网络,参与包括肿瘤在内的多种疾病的发生发展。本文综述了RNA甲基化修饰的类型、m5C甲基化检测技术的分类与进展及m5C动态调控网络参与肿瘤发生发展的最新研究进展,旨在为以RNA m5C调控网络为靶点的肿瘤治疗方案提供新思路。

As an important part of post transcriptional modification, RNA methylation mainly includes N6-methyladenosine (m6A), 5-methylcytosine (m5C) and N1-methyladenosine (m1A). The devel-opment of single base resolution detection technology represented by RNA-BisSeq has promoted the research of RNA m5C methylation modification. The regulatory network of RNA m5C methyla-tion modification, which is composed of methyltransferase, demethylase and methylation recogni-tion protein, is involved in the occurrence and development of a variety of diseases, including tu-mors. This paper reviews the types of RNA methylation modification, the classification and progress of m5C methylation detection technology and the latest research progress of dynamic regulatory network involved in tumorigenesis and development, in order to provide new ideas for tumor treatment targeting RNA m5C regulatory network.