TPD52L1在胃癌中的表达及其临床意义

Expression of TPD52L1 in Gastric Cancer and Its Clinical Significance

目的:探究肿瘤蛋白家族D52 (TPD52)的成员TPD52L1 (TPD53)蛋白在胃癌及胃癌细胞中的表达,并分析其表达与胃癌患者临床病理特征的相关性。方法:收集2020年01月至2022年06月期间青岛市市立医院普外科经手术完整切除的胃癌及癌旁(距肿瘤组织5 cm以上,病理证实无癌组织浸润)标本总共33例。采用免疫组织化学法检测TPD52L1在33例胃癌组织中的表达,分析TPD52L1表达水平与胃癌病例特征的相关性。通过RT-qPCR检测TPD52L1在胃癌细胞系及人胃粘膜上皮细胞系中的表达水平。结果:在胃癌组织中TPD52L1的蛋白表达水平明显高于癌旁组织。此外,在胃癌细胞中,TPD52L1的蛋白表达水平同样高于正常人胃粘膜上皮细胞。结论:TPD52L1在胃癌组织中的表达水平明显高于癌旁组织,其表达水平与胃癌患者的性别、淋巴结转移情况有关。此外,在胃癌细胞中,TPD52L1的表达同样高于正常人胃粘膜上皮细胞。

Objective: To investigate the expression of TPD52L1 (TPD53) protein, a member of the tumor pro-tein family D52 (TPD52), in gastric cancer and gastric cancer cells, and to analyze the correlation between its expression and clinicopathological characteristics in patients with gastric cancer. Methods: From January 2020 to June 2022, a total of 33 specimens of gastric cancer and its adjacent tissues (more than 5 cm away from the tumor tissue, pathologically confirmed without invasion of cancer tissue) were collected from the General Surgery Department of Qingdao Municipal Hospital. Immunohistochemical method was used to detect the expression of TPD52L1 in 33 cases of gastric cancer, and the correlation between the expression level of TPD52L1 and the characteristics of gas-tric cancer cases was analyzed. The expression level of TPD52L1 in gastric cancer cell lines and hu-man gastric mucosal epithelial cell lines was detected by RT-qPCR. Results: The protein expression level of TPD52L1 in gastric cancer tissue was significantly higher than that in adjacent tissues. In addition, the protein expression level of TPD52L1 in gastric cancer cells is also higher than that in normal human gastric mucosal epithelial cells. Conclusion: The expression level of TPD52L1 in gas-tric cancer tissue is significantly higher than that in adjacent tissues, and its expression level is re-lated to the gender and lymph node metastasis of gastric cancer patients. In addition, the expres-sion of TPD52L1 in gastric cancer cells is also higher than that in normal human gastric mucosal ep-ithelial cells.