鉴定克罗恩病和溃疡性结肠炎中共同的免疫相关基因

Identifying Common Immune-Related Genes in Crohn’s Disease and Ulcerative Colitis

目的:筛选出UC和CD患者的共有的表达异常的免疫相关基因,并比较UC和CD患者的免疫浸润的异同;方法:从GEO获取2个肠粘膜基因表达矩阵GSE87473和GSE102133,对其进行差异分析,以及WGCNA获取关键基因,并对获得的基因进行GO和KEGG分析,最后用CIBERSORT获取UC和CD患者的免疫浸润情况,并分析免疫相关基因与免疫细胞的相关性。结果:UC和CD患者中共有8个差异表达的免疫相关共同基因,分别是:DUOX2,LCN2,PI3,CXCL1,IDO1,STAT1,CXCL2和PLAUR。UC和CD患者具有相似的免疫细胞浸润结果和轻微的免疫细胞浸润差异。识别的免疫相关共同基因或多或少与同时失调的免疫细胞显著相关。结论:本研究利用生物信息学方法,筛选出8个UC和CD患者共有的表达异常的免疫相关基因,为深入研究IBD患者的发病机制、诊断以及靶向治疗带来了新的思路。

Objective: To screen for immune-related genes with abnormal expression common to patients withUC and CD and to compare the similarities and differences in immune infiltration in patients with UCand CD. Methods: Two intestinal mucosal gene expression matrices, GSE87473 and GSE102133,were obtained from GEO for differential analysis, as well as WGCNA to obtain essential genes andGO and KEGG analysis of the obtained genes, and finally, CIBERSORT was used to obtain immuneinfiltration in UC and CD patients and to analyze the correlation between immune-related genesand immune cells. Results: There were eight differentially expressed immune-associated commongenes in UC and CD patients: DUOX2, LCN2, PI3, CXCL1, IDO1, STAT1, CXCL2 and PLAUR. UC and CDpatients had similar immune cell infiltration results and slight differences in immune cell infiltration.Identified immune-related common genes were more or less significantly associated withconcurrently dysregulated immune cells. Conclusion: Using a bioinformatics approach, this studyscreened for eight immune-related genes with abnormal expression common to UC and CD patients,bringing new ideas for an in-depth study of pathogenesis, diagnosis and targeted therapy inIBD patients.