摘要
流式细胞术(flow cytometry, FCM)是七十年代发展起来的一项高学科技术,它不仅可以测量细胞大小、内部颗粒性状,还可对细胞表面、胞浆抗原、细胞内的核酸、细胞因子等进行定量、快速、客观、多参数相关检测。它是集光学、电子学、流体力学、细胞化学、物理学、生物学、免疫学、以及激光和计算机等多门学科和技术于一体的综合运用产物。流式细胞仪具有检测速度快、测量指标多、采集数据量大、分析全面、方法灵活等特点,随着其性能的不断改进和测定方法与技术的快速发展,FCM已成为某些疾病不可或缺的辅助诊断技术。本文就流式细胞术的原理及在各领域的应用、发展趋势进行综述。
Flow cytometry (FCM) is a high-tech technology developed in 1970s, which can not only measure cell size and internal particle properties, but also quantitatively, rapidly, objectively and mul-ti-parameter correlated detection of cell surface, cytoplasmic antigen, intracellular nucleic acid, cy-tokine, etc. It is a collection of optics, electronics, fluid mechanics, cytochemistry, physics, biology, immunology, as well as laser and computer and other disciplines and technologies in one compre-hensive application product. Flow cytometry has the characteristics of fast detection speed, many measurement indicators, large amount of data collection, comprehensive analysis and flexible method. With the continuous improvement of its performance and the rapid development of meas-urement methods and techniques, FCM has become an indispensable auxiliary diagnostic technol-ogy for some diseases. This paper reviews the principle, application and development trend of flow cytometry.
出处
《临床医学进展》
2023年第5期8355-8360,共6页
Advances in Clinical Medicine
