摘要
目的:分析乙醛脱氢酶1A1 (ALDH1A1)在肝细胞癌(HCC)与正常肝脏中的表达差异,探讨ALDH1A1在HCC诊断中的价值。方法:实时荧光定量PCR (qRT-PCR)和免疫印迹法(Western blot)分别检测人肝癌细胞株HepG2与人正常肝细胞株LO2中ALDH1A1 mRNA及蛋白的表达情况;免疫组织化学法(IHC)检测49例HCC及其癌旁组织中ALDH1A1的表达情况。结果:人肝癌细胞株HepG2中ALDH1A1 mRNA及蛋白的表达均明显高于人正常肝细胞株LO2 (P < 0.05, P < 0.0001);HCC组织中ALDH1A1的表达明显高于癌旁组织(P < 0.05)。结论:ALDH1A1在HCC中的表达升高,ALDH1A1有望作为诊断HCC的有效分子标志物。
Objective: To analyze the differential expression of acetaldehyde dehydrogenase 1A1 (ALDH1A1) in Hepatocellular carcinoma (HCC) and normal liver, and to explore the value of ALDH1A1 in the diag-nosis of HCC. Methods: qRT PCR and Western blot were used to detect the expression of ALDH1A1 mRNA and protein in HepG2 and LO2, respectively;IHC was used to detect the expression of ALDH1A1 in 49 HCC and paracancerous tissues. Results: The expression of ALDH1A1 mRNA and protein in HepG2 was significantly higher than that in LO2 (P < 0.05, P < 0.0001);The expression of ALDH1A1 in HCC tissue was significantly higher than that in paracancerous tissues (P < 0.05). Con-clusion: The expression of ALDH1A1 is elevated in HCC, and ALDH1A1 is expected to serve as an ef-fective molecular marker for the diagnosis of HCC.
出处
《临床医学进展》
2023年第7期11864-11869,共6页
Advances in Clinical Medicine
