甲状腺乳头状癌患者血清样本代谢组学分析

Metabolomics Analysis of Serum Samples from Patients with Papillary Thyroid Cancer

目的:分析对比甲状腺乳头状癌(PTC)及健康成人血清样本筛选差异代谢物和异常代谢通路,为PTC的早期诊疗提供新的思路。方法:选取2022年3月至2022年12月就诊于山东省青岛大学附属中心医院经病理确诊的PTC患者的血清样本30例,并收集健康成人血清样本36例,利用超高效液相色谱四极杆飞行时间质谱联用技术,采用主成分分析和偏最小二乘判别分析等方法进行数据处理,筛选差异相关的生物标志物和代谢通路。结果:通过分析筛选获得41种可信差异代谢物,其中22种下调,19种上调,在PTC中,α-溶血磷脂酸、花生四烯酸等呈下降趋势;顺乌头酸、α-亚麻酸等呈上升趋势。筛选出20条代谢通路,分别为氮代谢、赖氨酸降解通路、花生四烯酸代谢通路等。结论:PTC和健康成人组代谢轮廓存在显著差异;糖酵解、氨基酸代谢、脂代谢参与了PTC的病理过程。

Objective: To analyze and compare the differential metabolites and abnormal metabolic pathwaysin serum samples of papillary thyroid carcinoma (PTC) and healthy adults, and provide new ideasfor the early diagnosis and treatment of PTC. Method: Thirty serum samples of PTC patients diagnosedwith pathology at the Affiliated Central Hospital of Qingdao University in Shandong Provincefrom March 2022 to December 2022 were selected, and 36 healthy adult serum samples werecollected. The data were processed using ultra-high performance liquid chromatography quadrupoletime-of-flight mass spectrometry (UHPLC), principal component analysis (PCA), partial leastsquares discriminant analysis (PLSA), and differentially correlated biomarkers and metabolic pathwayswere screened. Result: 41 credible differential metabolites were obtained through analysis andscreening, of which 22 were down regulated and 19 were up regulated. In PTC, α-Lysophosphatidicacid and arachidonic acid showed a downward trend;Shunaconitic acid α-Linolenic acid and othersubstances are showing an upward trend. 20 metabolic pathways were screened, including nitrogenmetabolism, lysine degradation pathway, arachidonic acid metabolism pathway, etc. Conclusion:There is a significant difference in metabolic profile between PTC and healthy adult groups;glycolysis, amino acid metabolism, and lipid metabolism are involved in the pathological processof PTC.