摘要
研究酒精丁香提取物(Alcohol Extract of Clove, AEC)对人源性卵巢癌细胞A2780增殖抑制和周期的影响及其潜在的作用机制。方法:CCK8法检测A2780卵巢癌细胞经AEC作用后的增殖情况;克隆形成检测A2780细胞群体依赖性情况,软琼脂克隆法检测AEC作用后的成团能力;Transwell试验检测与划痕实验细胞迁移情况;流式细胞术检测细胞周期情况。结果:CCK8实验结果提示AEC具有抑制卵巢癌细胞的增殖活性,并呈浓度依赖性;平板克隆与软琼脂克隆结果说明了AEC抑制卵巢癌细胞的生长;划痕实验和Transwell实验结果显示AEC作用卵巢癌细胞后抑制了迁移能力卵巢癌细胞经AEC处理后显微镜观察细胞形态发生变化并促进细胞的凋亡。流式细胞周期结果显示AEC可以引起G1期和S期增多。结论:AEC对卵巢癌细胞系有增殖抑制的作用,抑制细胞迁移并促进卵巢癌的凋亡并改变卵巢癌细胞的形态,将周期抑制在G1期和S期。
Exploring the potential mechanism of action of alcoholic extract of clove (AEC) in inhibiting proliferation and cycling of human ovarian cancer A2780 cells, this paper seeks to investigate its effect. The CCK8 assay indicated that AEC had a concentration-dependent effect on ovarian cancer cell proliferation;plate cloning and soft agar cloning revealed that AEC inhibited ovarian cancer cell growth;and the scratch assay and Transwell assay demonstrated that AEC had a hindrance on ovarian cancer cell migration following AEC treatment. The results of cell cycle analysis showed that AEC induced growth in G1 and S phases. To sum up, AEC hinders the growth of ovarian cancer cell lines, obstructs cell movement, encourages apoptosis, modifies the shape of ovarian cancer cells, and impedes the G1 and S phases of the cell cycle.
出处
《临床医学进展》
2024年第4期2330-2339,共10页
Advances in Clinical Medicine
