扶正散结方对肺腺癌Bax、Bcl-2表达及肿瘤生长的影响

Effects of Fu Zheng San Jie Fang on Bax and Bcl-2 Expression and Tumour Growth in Lung Adenocarcinoma

目的:观察扶正散结方对肺腺癌Bax、Bcl-2表达及肿瘤生长的作用效果。方法:建立肺腺癌H1299细胞裸鼠移植瘤模型,将20只荷瘤鼠随机分成模型对照组(后简称对照组)和扶正散结方干预组(2.275 g/mL) (后简称扶正散结方组)、扶正散结方联合阳性药物替吉奥干预组(后简称联合组)及阳性药物替吉奥对照组(后简称阳照组),每1 d灌胃给药1次,于每次给药前测定各组荷瘤鼠体质量,药物干预21 d后处死小鼠,剥离瘤体并称量瘤体质量,采用免疫组织化学染色检测不同处理组细胞Bax、Bcl-2表达水平。同时体外培养H1299细胞,应用细胞计数试剂盒(CCK-8)检测不同浓度扶正散结方对细胞增殖的影响。结果:与对照组[(22.8 1.0) g]比较,联合组[(19.10 ± 1.18) g]、阳照组[(20.10 ± 0.17) g]干预21 d后小鼠体质量增长均有明显下降(P P P < 0.05)。各浓度扶正散结方均可对体外培养的H1299细胞的增殖产生抑制效果,且此抑制效果随药物浓度增加与干预时间延长而提高。结论:扶正散结方对肺腺癌生长具有抑制作用,其可通过上调促凋亡蛋白,下调抗凋亡蛋白的表达达成上述目标。同时体外实验也证明,扶正散结方对肺腺癌H1299细胞的增殖具有抑制作用。即扶正散结方可通过促进凋亡及抑制增殖两方面实现抑制肿瘤生长。

Objective: To observe the effects of Fu Zheng San Jie Fang on the expression of Bax and Bcl-2 and tumour growth in lung adenocarcinoma. Methods: A lung adenocarcinoma transplantation model was established in nude mice with H1299 cells, and 20 tumor-bearing mice were randomly divided into a model control group (later referred to as the control group), an intervention group of Fu Zheng San Jie Fang (2.275 g/mL) (later referred to as the group of Fu Zheng San Jie Fang), an intervention group of Fu Zheng San Jie Fang combined with a positive drug, Tegio (later referred to as the combined group), and a control group of a positive drug, Tegio (later referred to as the positive control group), and the drug was administered by gavage once every 1 d. The body mass of rats in each group was measured before each administration of the drug, and the mice were executed after 21 d of drug intervention, the tumours were stripped and weighed, and the expression levels of Bax and Bcl-2 in the cells of different treatment groups were detected by immunohistochemical staining. At the same time, H1299 cells were cultured in vitro, and a cell counting kit (CCK-8) was applied to detect the effects of different concentrations of Fu Zheng San Jie Fang on cell proliferation. Results: Compared with the control group [(22.8 1.0) g], there was a significant decrease in the growth of body mass of mice in the combined group [(19.10 ± 1.18) g] and the Yang-photographed group [(20.10 ± 0.17) g] after 21 d of the intervention (P P P in vitro, and this inhibitory effect increased with the increase of drug concentration and the prolongation of intervention time. Conclusion: Fu Zheng San Jie Fang can inhibit the growth of lung adenocarcinoma by up-regulating the expression of pro-apoptotic proteins and down-regulating the expression of anti-apoptotic proteins. Meanwhile, in vitro experiments also proved that Fu Zheng San Jie Fang can inhibit the proliferation of lung adenocarcinoma H1299 cells. In other words, Fu Zheng San Jie Fang can inhibit tumour growth by promoting apoptosis and inhibiting proliferation.