高糖条件下HRCECs中GALNT2与p-EGFR相关性研究

A Study on the Correlation between GALNT2 in HRCECs and p-EGFR under High Sugar Conditions

目的:探讨在高糖条件下敲低多肽N-乙酰半乳糖胺转移酶2 (GALNT2)与抑制磷酸化表皮生长因子受体(Phosphorylated epidermal growth factor receptor, p-EGFR)对视网膜血管内皮细胞(HRCECs)增生及凋亡的影响。方法:本研究以高糖(25 mmol·L−1)状态下体外培养的HRCECs为研究对象,将HRCECs分为mock组(25 mmol·L−1葡萄糖)、mock + shGALNT2组(25 mmol·L−1葡萄糖 + GALNT2敲低病毒)、mock + NC-shGALNT2 + Chrysophanol组(25 mmol·L−1葡萄糖 + 对照病毒 + Chrysophanol)、mock + shGALNT2 + Chrysophanol组(25 mmol·L−1葡萄糖 + GALNT2敲低病毒 + Chrysophanol),连同培养液在37℃恒温培养箱中培养24 h,比较各组GALNT2 mRNA相对表达量、细胞增生值、细胞凋亡率,以及GALNT2、EGFR、EGF、p-EGFR蛋白相对表达量。结果:mock组、mock + shGALNT2、mock + NC-shGALNT2 + Chrysophanol组和mock + shGALNT2 + Chrysophanol组细胞中GALNT2 mRNA相对表达量比较,差异有统计学意义(P P P F = 96.56, P P P P P > 0.05)。结论:在高糖条件下,敲低的GALNT2可以通过介导p-EGFR可以提高HRCECs细胞的增殖能力,减少HRCECs细胞的凋亡。

Objective: To investigate the effects of knocking down peptide N-acetylgalactosamine transferase 2 (GALNT2) and inhibiting phosphorylation of epidermal growth factor receptor under high glucose conditions on the proliferation and apoptosis of retinal vascular endothelial cells (HRCECs). Method: This study focuses on cultured HRCECs in vitro under high glucose (25 mmol·L−1) conditions, and divides them into a mock group (25 mmol·L−1 glucose), mock + shGALNT2 group (25 mmol·L−1 glucose + GALNT2 knockdown virus), mock + NC-shGALNT2 + Chrysophanol group (25 mmol·L−1 glucose + control virus + Chrysophanol), mock + shGALNT2 + Chrysophanol group (25 mmol·L−1 glucose + GALNT2 knockdown virus + Chrysophanol) and culture medium were cultured in a constant temperature incubator at 37˚C for 24 hours. The relative expression level of GALNT2 mRNA, cell proliferation value, apoptosis rate, GALNT2, EGFR, EGF, and p-EGFR proteins were compared between the groups. Results: The relative expression levels of GALNT2 mRNA in the cells of the mock group, mock + shGALNT2 group, mock + NC-shGALNT2 + Chrysophanol group, were compared with mock + shGALNT2 + Chrysophanol group, and the differences were statistically significant (P P P P P P P P > 0.05). Conclusion: Under high glucose conditions, knocking down GALNT2 can enhance the proliferation ability of HRCEC cells and reduce apoptosis by mediating p-EGFR.