生长分化因子15抑制心肌梗死大鼠细胞凋亡的实验研究

Experimental Study on Inhibition of Apoptosis by Growth Differentiation Factor 15 in Rats with Myocardial Infarction

目的:探讨生长分化因子15对心肌梗死大鼠的作用。方法:18只SPF级雄性SD大鼠根据随机数字表法分为模型组、实验组(A组GDF-15低剂量组10 ng/mL、B组GDF-15高剂量组50 ng/mL),每组6只,适应性喂养1周后,每天给药1次,连续1周,末次给药6 h后,各组大鼠均结扎冠状动脉左前降支进行心肌梗死造模。采用TUNEL/DAPI双染色法观察大鼠心肌细胞凋亡情况,分别统计凋亡细胞数及细胞总数,计算出细胞凋亡率。结果:与模型组相比,对照组B的细胞凋亡率显著减少(P Objective: To investigate the effect of growth differentiation factor 15 on myocardial infarction in rats. Methods: 18 SPF grade male SD rats were divided into model group and experimental group according to random number table method (group A, low-dose GDF-15 group, 10 ng/mL, group B, high-dose GDF-15 group, 50 ng/mL), with 6 rats in each group. After adaptive feeding for 1 week, the rats were given the drug once a day for 1 week. The left anterior descending branch of the coronary artery was lapped in each group for myocardial infarction modeling. TUNEL/DAPI double staining was used to observe the apoptosis of rat cardiomyocytes. The number of apoptotic cells and the total number of apoptotic cells were counted, and the apoptosis rate was calculated. Results: Compared with model group, the apoptosis rate of control group B was significantly decreased (P < 0.01), and GDF-15 reduced the apoptosis rate in a dose-dependent manner (P < 0.05). Conclusion: Growth differentiation factor 15 can reduce apoptosis in rats with myocardial infarction.