OSMAC策略在费希新萨托菌次级代谢产物研究中的应用

Study on the Secondary Metabolites of Neosartorya fischeri via the OSMAC Method

本研究选取根据基因预测结果存在大量生物合成基因簇但目前次级代谢产物研究的仍较少的费希新萨托菌(Neosartorya fischeri NRRL 181)作为研究对象,先利用OSMAC策略研究了不同培养基下费希新萨托菌的次级代谢产物情况,再选择了大米作为培养基对该菌进行了大规模发酵。发酵结束后对95%乙醇提取所得的浸膏利用传统的柱层析手段进行了系统的次级代谢产物分离工作,共分离并鉴定了8个化合物。

It seems that Neosartorya fischeri NRRL 181 has a large number of biosynthetic gene clusters based on the predicting results of gene. But its secondary metabolites are still less studied at present. In this study, the OSMAC strategy was used to study the secondary metabolites of N. fischeri in different media. Then the rice was selected as the medium for large-scale fermentation. After fermentation, the extract obtained from 95% ethanol was used to separate the secondary metabolites using traditional column chromatography. A total of 8 compounds were separated and identified.