降解造纸废水微生物絮凝剂产生菌的筛选及鉴定分析

Isolation, Identification and Characteristics of a Bioflocculant-Producing Bacterium for Degradation of Papermaking Wastewater

从造纸厂沉淀池活性污泥中分离得到1株产絮凝剂菌株A2。经鉴定,该菌株为杆状革兰氏阳性菌,为Alcaligenes faecalis subsp. phenolicus。该菌株在添加有葡萄糖、木质素及H酸的培养基中,均能产生絮凝剂。通过对絮凝剂进行纯化,使用红外光谱对絮凝剂的表征进行初步分析,发现絮凝剂中存在OH、NH2、O=C-NH基团。添加不同的碳源研究其絮凝效果,发现该菌株在葡萄糖、木质素和H酸中都有较好的絮凝效果。实验结果表明,该菌株具有较高降解造纸厂废水的能力。

A flocculant producing strain A2 was isolated from activated sludge of settling tank in paper mill. The strain was identified as Alcaligenes faecalis subsp. phenolicus. The strain can produce flocculant in the medium with glucose, lignin and H acid. By purifying the flocculant and preliminarily analyzing the characterization of the flocculant with infrared spectrum, it is found that there are OH, NH2, O=C-NH groups in the microbial flocculant. Adding different carbon sources to study the flocculation effect, it was found that the strain had good flocculation effect in glucose, lignin and H acid. The experimental results showed that the strain had high degradation ability of paper mill wastewater.