中药黄芩煮沸液的荧光光谱

The Fluorescence Spectra of the Boiling Liquid of Scutellaria baicalensis Georgi

利用日本岛津(Shimadzu)RF-5301PC荧光光度计,研究了激发波长在450~530nm内黄芩煮沸液的三维荧光光谱,以及荧光强度与浓度、温度之间的关系。实验发现煮沸液中主要存在一种荧光组分,该组分最佳激发波长为λexλem=492nm/534nm;荧光强度随着黄芩煮沸液浓度的增大逐渐增强,荧光强度与浓度之间所得回归方程为y=0.213x+145.68,线性相关系数为R2=0.9746;荧光强度随着温度的增大而逐渐减小,荧光峰位置未发生改变,荧光强度与温度回归方程为y=?2.3428x+442.89,线性相关系数为R2=0.9976。实验表明,黄芩溶液中可能存在一种荧光型体或荧光组分,随温度的增大,荧光型体荧光强度降低。本实验为黄芩的定性检测分析提供了实验依据,对光诱导荧光光谱诊断技术中激发光波长的选择具有一定的参考价值。

The fluorescence spectra of the three-dimensional fluorescence spectra of the boiling liquid of Scutellaria baicalensis Georgi in 450 - 530 nm and the relationship between the fluorescence intensity and the concentration and temperature were studied,with the fluorescence photometer RF5301 (SHIMADZU) made in Japan. It’s found that there is a kind of fluorescent component in the boiling liquid,and the best excitation wavelength is 492 nm/534 nm. The fluorescence intensity increased with the concentration of Scutellaria baicalensis Georgi boiling. The regression equation between fluorescence intensity and concentration was y = 0.213x + 145.68,and the linear correlation coefficient was R2 = 0.9746. The fluorescence intensity decreased with the increase of temperature,and the location of fluorescence peak did not change. The regression equation between fluorescence intensity and temperature was y = ?2.3428x + 442.89,and the linear correlation coefficient was R2 = 0.9976. The results show that there may be a fluorescent or fluorescence component in the baicalein solution. With the increase of temperature,the fluorescence intensity of the fluorophore is reduced. This experiment provides experimental basis for qualitative detection and analysis of Scutellaria baicalensis Georgi,and has a certain reference value for the selection of excitation wavelength in the diagnostic technology of light induced fluorescence.