飞秒荧光光谱技术及其在生命科学中的应用

Femtosecond Fluorescence Spectroscopy and Its Applications in Life Science

频率上转换荧光光谱技术在生物大分子的结构、功能以及动力学研究方面具有广泛的应用。近年来,随着超快激光技术的发展以及相关光电子设备的升级和更新,尤其是飞秒激光的出现,频率上转换技术的时间分辨率达到了飞秒量级,为生物、化学和医学等领域的研究带来了新的发展契机。本文介绍和回顾了频率上转换荧光光谱技术的基本原理,在自行组建的一套频率上转换飞秒荧光光谱实验系统基础上,重点分析了整体系统的关键设计因素。介绍了频率上转换飞秒荧光光谱技术在生命科学领域,尤其是蛋白质、DNA动力学研究中的一些应用。

Frequency upconversion fluorescence spectroscopy can be widely used to study biological macromolecules’ structures and dynamics which may be linked to their functions. With the developments of ultrafast laser technology and the updates of related optoelectronic devices, the presence of femtosecond laser especially makes the time resolution of frequency upconversion down to femtosecond, which provides new opportunities to the studies in biology, chemistry and medicine science. In this paper, the basic principle of upconversion technique is briefly reviewed. On the basis of a self-constructed femtosecond upconversion fluorescence spectroscopy experimental system, the key design features of the total system are discussed. Finally, a few applications of the upconversion technique in life science, especially the fluorescence dynamics of proteins and DNA, are demonstrated.