蔗糖与接种量组合对长春花培养细胞吲哚生物碱合成的影响

Effect of Sucrose and Inoculating Quantity Combination on the Indole Alkaloids Synthesis of Catharanthus Roseus Culture Cell

实验结果表明,初始pH为6.5时有利于细胞生物量积累和吲哚生物碱的合成。接种量在20%时,随着蔗糖浓度的提高吲哚生物碱的含量也提高,添加90 g/L蔗糖时,长春碱的含量达到了1.7 mg/g干细胞。当接种量提高到25%或30%时,较低浓度的蔗糖有利于吲哚生物碱的合成。60 g/L蔗糖与25%接重量组合有利于细胞生物量的积累。

The experimental results show that, the initial pH of 6.5 is the optimum condition to the accumulation of cell biomass and the synthesis of indole alkaloids. When inoculation rate is at 20%, the content of indole alkaloids increase with the sucrose concentration increased, add 90 g/L sucrose, the content of vinblastine reached 1.7 mg/g dry cell. When the inoculation rate increased to 25% or 30%, a lower concentration of sucrose is conducive to the synthesis of indole alkaloids. 60 g/L sucrose and 25% of the inoculation rate combination is conducive to the cell biomass accumu- lation.