中缅树鼩PRDM16、BMP7、PPARα、COX II及PGC-1α基因的扩增研究

Amplification of PRDM16, BMP7, PPARα, COX II and PGC-1α Genes Tupaia belangeri

本研究对中缅树鼩PRDM16、BMP7、PPARα、COX II及PGC-1α基因的部分序列进行扩增和分析,获得中缅树鼩PRDM16、BMP7、PPARα、COX II及PGC-1α片段分别为184 bp、84 bp、358 bp、212 bp、271 bp,推测其分别编码61、27、119、70、87个氨基酸。将PRDM16、BMP7、PPARα、COX II及PGC-1α基因编码氨基酸序列分别与多种哺乳动物对应的氨基酸序列进行同源性比较分析发现氨基酸的同源性较高。以获得的PRDM16、BMP7、PPARα、COX II及PGC-1α基因序列分别构建系统进化树,结果显示中缅树鼩与灵长类动物的亲缘关系较近。

In the present study, partial cDNA sequence of PRDM16, BMP7, PPARα, COX II and PGC-1α genes in Tupaia belangeri were amplificated and analyzed. The obtained partial sequences of PRDM16, BMP7, PPARα, COX II and PGC-1α in T. belangeri were 184 bp, 84 bp, 358 bp, 212 bp and 271 bp, respectively. The coding protein of these sequences included 61 amino acids in PRDM16;27 amino acids in BMP7;119 amino acids in PPARα, 70 amino acids in COX II and 87 amino acids in PGC-1α. Comparison of the amino acids of PRDM16, BMP7, PPARα, COX II and PGC-1α with the amino acids in many mammals found that the homology of amino acids was higher. The PRDM16, BMP7, PPARα, COX II and PGC-1α genes were used to reconstruct phylogenetic trees which showed that T. belangeri had the closest relationship relative to primates.