GC-MS/MS法分析水稻叶子单萜倍半萜类化合物的组成

Analysis of Mono(sesqui)terpenes by GC-MS/MS in Rice Leaves

以正己烷为提取溶剂,采用气相色谱–质谱联用法(GC-MS)鉴定出水稻三叶期叶片6种单萜类化合物和2种倍半萜类化合物。针对鉴定出来的8种化合物建立靶向定量代谢组方法,采用多反应监测(MRM)的质谱方法对水稻三叶期叶片中的6种单萜类物质及2种倍半萜类物质进行定量分析。结果表明,倍半萜trans-金合欢醇的含量在这8种化合物中含量最高(10359.1 ±117.7ng?g?1 FW),同时也是水稻叶子中单萜倍半萜类化合物的主要组成成分。

Six monoterpenes and two sesquiterpenes were identified by GC-MS from rice leaves using hexane as solvent. To quantitatively analyze these eight compounds, we established a target method (MRM) for these six monoterpenes and two sesquiterpenes in rice leaves. Results showed that sesquiterpene trans-farnesol was the major compounds of these volatile terpenes and the fresh weight of trans-farnesol reached to 10359.1 ±117.7 ng?g?1 FW.