摘要
利用AFLP分子标记技术对山东百部4个居群120个样品进行了遗传多样性分析。8对引物组合共扩增出282条清晰可辨条带,其中多态性条带137条,多态性位点百分率48.58%;在种级水平上,Nei’s基因多样性指数为0.1441,Shannon多态性信息指数为0.2485;居群总遗传多样性(Ht)为0.1646,居群内遗传多样性为0.1682,基因分化系数为0.0210。UPGMA聚类表明,AFLP聚类结果与表型分化聚类结果一致,居群间具有一定程度的遗传分化,并通过表型性状表现出来。
Genetic diversity and genetic structure of 120 individuals of four Stemona shandongensis popula- tions were investigated by AFLP method. A total of 282 bands were observed with 8 primer combination, in- cluding 137 polymorphic bands. The percentage of polymorphic bands was 48.58%. In species level, Nei’s gene diversity index was 0.1441;Shannon’s information index was 0.2485;total gene diversity was 0.1646;population gene diversity was 0.1682, gene differentiation coefficient was 0.0210. UPGMA cluster based on AFLP makers was similar to the cluster based on morphological traits;there was no significant correlation between genetic distance and geographic distance. The results showed that there was genetic differentiation among populations, and it could express by morphological characteristics.
出处
《植物学研究》
2013年第1期1-5,共5页
Botanical Research
基金
山东省自然科学基金(Y2006D12)
山东省农业良种工程重大课题(鲁农良字[2010]6号)。
