烟草BY-2原生质体定向伸长对离心力响应的相关性研究

The Correlation Studies on the Responses of Directional Elongation to a Centrifugal Force in Tobacco BY-2 Cells

本研究以烟草BY-2细胞为材料,在单细胞水平探讨应力条件对植物细胞定向伸长的影响。将烟草原生质体包埋于琼脂糖凝胶块中,通过施加不同大小的离心力和在恒定的施力条件下处理不同时间分析应力与细胞定向伸长的相关性。结果表明,在恒定施力条件下,原生质体的定向伸长与施力时间密切相关,倾向以垂直于应力方向伸长,且细胞的定向伸长与应力强度存在剂量关系。细胞微管骨架与离心力的方向平行。

In this study, the protoplasts from tobacco BY-2 (Nicotiana tabacum) were used to determine whether cells can be induced to expand in a preferential direction in response to an externally applied centrifugal force. Spherical protoplasts were embedded into agarose block. Constant uniaxial pressure released by a centrifugal force was imposed on the block. The results showed that protoplasts elongated with a preferential axis oriented perpendicularly to the applied force direction, according to a certain dose-dependent relation between the intensity of centrifugal force and the stress induced responses. The microtubules with centrifuged protoplasts were found to be oriented parallel to the centrifugal force direction.