运用SCAR和CAPS分子标记辅助筛选辣椒恢复系研究

Using the SCAR and CAPS Markers Assisted Selection Pepper Restorer Research

以辣椒(Capsicum annuum L.)幼嫩叶片为研究材料,利用3对显性SCAR标记和1对共显性CAPS标记对16份已知育性的辣椒材料进行引物可行性验证,再利用这4对引物对112份未知恢复基因资源的辣椒材料进行恢复基因鉴定。结果表明:SCAR和CAPS在16份辣椒材料中的结果完全一致,112份未知恢复基因辣椒资源鉴定中有104份材料在4对引物检测结果完全一致,其中51份具有恢复基因连锁的特异带型,占比49.04%,且22份具有纯合的恢复基因。本研究将SCAR和CAPS分子标记结合起来建立了与恢复基因Rf有关的PCR检测方法,可对辣椒恢复基因及其等位基因纯合状态进行分子标记辅助筛选。

Pepper (Capsicum annuum L.) tender leaves were used as the research object in this present study. Three pairs of dominant SCAR markers and a pair of co-dominant CAPS markers were applied to detect sixteenth known sterility pepper materials for verifying the feasibility of these primers. Then, the four pairs of primers were used to identify restorer genes in one hundred and twelve unknown pepper materials. The result showed that SCAR and CAPS displayed completely consistent in the sixteenth pepper materials, which were known contain or uncertain restorer gene peppers. In the one hundred and twelve unknown restorer genes pepper resources, one hundred and four materials were identical in the four pairs of primers detection results, including fifty-one had dominant restorer genes, accounted for 49.04%, and twenty-two materials had homozygous restoring genes. SCAR and CAPS molecular markers were combined and established a restorer gene PCR detection method in this present study, which can be used in marker-assisted selection restoring genes and alleles homozygous state.