外源激素不同配比对“白草莓”茎尖萌发及增殖的影响研究

Effect of Different Ratio of Exogenous Hormones to the Tip Meristem Generation and Propagation of Pine-Berry

以日本白草莓为试材,研究6-BA浓度梯度及其它激素的配比不同对白草莓茎尖萌发和增殖的影响,结果表明,高浓度6-BA对茎尖萌发有抑制作用,对连续增殖也有不利影响,主要是矮化簇生和畸形;MS培养基加入6-BA 0.5 mg/L和NAA 0.1 mg/L,茎尖生长点萌发时愈伤化严重,且萌发率不高;MS培养基添加6-BA 0.5 mg/L和IBA 0.1 mg/L,“白草莓”茎尖萌发率为99%。第一代增殖MS培养基中添加6-BA 1.5 mg/L,IBA 0.1 mg/L,以后应逐渐降低6-BA浓度,直到降到0.5 mg/L,但IBA浓度保持0.1 mg/L不变,茎尖萌发苗增殖迅速,繁殖系数2.5以上。增殖的白草莓幼苗继代到6-BA 0.5 mg/L和NAA 0.1 mg/L的MS培养基上,长势迅速且健壮。

This paper researches about the effect of different ratio of exogenous hormones to the tip meristem generation and propagation of Japanese pine-berry which was introduced from Japan. 6-BA concentration gradient, hormone ratio, generation and reproduction efficiency were investigated. The results showed that, high concentration of 6-BA can retard the tip stem generation, and has negative effect on propagation. The main effects are dwarf, cluster, and abnormality. On the MS media with 6-BA 0.5 mg/L and NAA 0.1 mg/L, tip stem generating ratio was low and accompanied with callus occurred severely. However, on the MS media with 6-BA 0.5 mg/L and IBA 0.1 mg/L, the generation ratio was 99% in this experiment. First regeneration MS media with 6-BA 1.5 mg/L and IBA 0.1 mg/L, from second regeneration, the 6-BA concentration was gradually decreased to 0.5 mg/L in the end, but the IBA concentration remained unchanged. Under this condition, the generating seedlings regenerated rapidly. The propagation coefficient was higher than 2.5. Propagating seedlings sub-cultured on the MS media with 6-BA 0.5 mg/L and NAA 0.1 mg/L, grew rapidly and healthily.