抗寒大岩桐快速繁殖体系的建立

The Establishment of the Rapid Propagation System of Cold Resistance Gloxinia

[目的]:观赏花卉大岩桐为多年生球根草本花卉,但其种子繁殖困难且不耐寒。因此,研究抗寒大岩桐快速繁殖体系非常有必要。[方法]:山东农业大学王晓云教授实验室刚获得转耐寒基因大岩桐植株,利用该实验材料,建立了耐寒大岩桐的快速繁殖体系。[结果]:发现以小芽带小叶为外植体比以叶片和茎段为外植体出芽多,以MS + 15 g/L糖 + 0.1 mg/L NAA为增殖培养基比MS + 15 g/L糖 + 0.2 mg/L NAA或者MS + 15 g/L糖 + 0.1 mg/L NAA + 0.5 mg/L 6-BA增殖速度快。并且,以1/2MS + 30 g/L糖 + 0.2 mg/L NAA为生根培养基,最易在短时间内获得大量生根植株。[结论]:带有AtCBF1抗寒调控基因的转基因大岩桐最佳快速繁殖体系为以小芽带小叶为外植体,以MS + 15 g/L糖 + 0.1 mg/LNAA为增殖培养基。

[Objective]: The ornamental flower Gloxinia is a perennial bulbous herb, but its seeds are difficult to reproduce and barely cold-resista. So it is necessary to study the rapid propagation system of cold-resistant Gloxinia. [Methods]: Laboratory of Professor Wang Xiaoyun in Shandong Agricultural University has just obtained transgenic Gloxinia with cold tolerance gene. By using this experimental material, a rapid propagation system of cold tolerant Gloxinia was established. [Results]: The results showed that the explants with small buds and lobules produced more buds than those with leaves and stems. MS with 15 g/L sugar and 0.1 mg/L NAA as the proliferation medium was better than MS + 15 g/L sugar + 0.2 mg/L NAA or MS + 15 g/L sugar + 0.1 mg/L NAA + 0.5 mg/L 6-BA. The rooting medium with 1/2MS + 30 g/L sugar + 0.2 mg/L NAA was the shortest rooting medium. [Conclusion]: The optimum rapid propagation system of transgenic Gloxinia with AtCBF1 cold tolerance regulation gene is MS + 15 g/L sugar + 0.1 mg/L NAA medium with small buds and lobules as explants.