阳春砂叶片受病害诱导产生更多的挥发性萜类

Leaves of Amomum villosum Lour. Produce More Volatile Terpenoids Induced by Disease

目的:分析阳春砂病变叶片中的挥发性萜类,探究阳春砂叶片受病害刺激后挥发性萜类代谢的响应情况,为阳春砂抗病相关的萜类代谢及其抗病机理的研究奠定基础。方法:分别提取阳春砂病变叶片中健康部位和病变部位的挥发性萜类,采用GC-MS方法进行分析。结果:阳春砂叶片病变部位能检测到更多的挥发性萜类,有10种单萜和1种倍半萜仅在病变部位检测到,如葑烯、香芹醇、龙脑烯醛等,大多为含氧单萜。与健康部位相比,侧柏烯、莰烯、柠檬烯、松香芹醇的含量有显著性提高(p <0.05),其中柠檬烯的含量有极显著性提高(p <0.01),是健康部位的5.27倍。结论:阳春砂叶片受病害诱导产生更多的挥发性萜类,其种类增加,部分含量显著提高,这些挥发性萜类可能参与了阳春砂对病害的防御。

Objective:To analyze the volatile terpenoids composition in the pathological leaves of Amomum villosum Lour.and to explore the response of metabolism of volatile terpenoids while the leaves are stimulated by pathogenic bacteria.Method:Volatile terpenoids in the healthy and diseased parts of pathological leaves of A.villosum were extracted respectively and detected by GC-MS.Result:More species of volatile terpenoids were detected in the diseased part,including ten monoterpenoids and one sesquiterpenoid,as fenchene,carveol,campholenal etc.,most of which were oxygenated monoterpenoids.