甘蓝型油菜利油杂3号杂种纯度鉴定

Hybrid Purity Identification of Liyouza No. 3 in Bassica napus

本试验以甘蓝型油菜利油杂3号及其亲本(不育系T21、恢复系P12-7)为供试材料,在43对SSR引物中筛选出2对条带清晰且均为共显性的SSR引物(BN12A、CB10099)用于利油杂3号种子纯度鉴定。BN12A在137株杂交种样本出现父母本特异互补的杂交种带型共117株,父本带型4株,母本带型16株,杂交种纯度为85.40%;CB10099则出现父母本特异互补的杂交种带型共121株,父本带型2株,母本带型14株,杂交种纯度为88.32%。比较2对引物纯度鉴定结果,两者均存在单一引物鉴定为母本或父本,而另一引物鉴定为杂交种的现象。综合二者的带型结果杂交种带型共125株,父本带型1株,母本带型11株,杂交种纯度为91.24%。父本带型株与杂交种带型株总计比例为91.97%,与大田调查结果93%基本一致。说明该批次甘蓝型油菜利油杂3号杂种纯度符合商品种质量标准。同时,利用分子标记进行室内杂种纯度鉴定时,至少需要有两对及以上SSR标记扩增的结果进行综合评价。本研究可为其它甘蓝型油菜杂交种建立分子标记纯度鉴定技术体系提供有效的鉴定。

In this experiment,Liyouza No.3 and its parents(sterile line T21,restorer line P12-7)were used as experimental materials.Two pairs of primers(BN12A,CB10099)whose bands were clear and co-dominant were selected in 43 pairs of SSR primers and could be used for the purity identification of Liyouza No.3.There are 4 male plants,16 female plants and 117 hybrids with parental-specific complements in 137 hybrids about BN12A,with a purity of 85.40%.CB10099 also shows 121 hybrids with parental-specific complements in 137 hybrids,2 male plants and 14 female plants,with the hybrid purity identification of 88.32%.Comparing the results of two pairs of primer purity identification,both of them have the phenomena that one primer is identified as female parent or male parent,and the other primer is identified as hybrid.The hybrids of the combining bands of two primers are 125,1 male plant and 11 female plants,and the hybrid purity identification is 91.24%.The total ratio of the male-type plants and hybrid-type plants is 91.97%,which is close to the field purity identification result(93%).It indicates that the purity of Liyouza No.3 is in accordance with the quality standards of commercial varieties.At the same time,when using molecular markers for the identification of indoor hybrid purity,we need two pairs of SSR markers at least for comprehensive evaluation.This study can provide effective identification for the establishment of molecular marker purity identification technique systems for other hybrids of Brassica napus.