红景天愈伤组织诱导及红景天苷积累的研究

Study on Callus Induction and Salidroside Accumulation in Rhodiola L.

通过对红景天叶片、茎、根产生愈伤组织的培养,建立了培养周期短、愈伤组织生长效率高的培养体系。利用多因子正交实验设计及分析,筛选出最佳愈伤组织诱导培养基是MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA + 0.1 mg/L 2,4-D。通过甲基茉莉酸的添加研究诱导愈伤组织生物量及药效物质积累的影响,发现甲基茉莉酸浓度对愈伤组织生物量及药效物质红景天苷积累有显著影响,其中25.0 mg/L MeJA对愈伤组织生物量及红景天苷的积累效果最好。因此,通过培养愈伤组织是一种获得大量红景天活性物质的有效方法,为红景天产品的生产提供一种原材料途径。

A highly effective and rapid culture system of callus was developed by cultivating callus from leaves, stems and roots of Rhodiola L. The design and analysis of multivariate experiments showed the optimum medium for callus induction was MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA + 0.1 mg/L 2,4-D. The effects of methyl jasmonate (MeJA) on callus biomass and bioaccumulation of salidroside were studied by adding MeJA. It was found that the concentration of MeJA had significant effects on callus biomass and bioaccumulation of salidroside, of which 25.0 mg/L MeJA had the best effect on callus biomass and accumulation of salidroside. Therefore, callus culture is an effective way to obtain a large number of active substances of Rhodiola, which provides a raw material for the production of Rhodiola products.