植物转录因子研究方法

Research Method of Transcription Factors in Plant

植物转录因子可以调控众多下游基因的表达,在植物的生长发育、响应外界环境刺激等方面起着重要的调控作用。随着转录因子在模式植物和作物中基因转录调控的研究不断深入,转录因子研究方法也在持续创新。我们主要从蛋白质与蛋白质互作和靶基因与蛋白质互作这2个方面阐述植物转录因子研究方法,为植物转录因子的相关研究提供参考方法。蛋白质与蛋白质互作的方法主要有:酵母双杂交系统、荧光共振能量转移技术、双分子荧光互补技术、下拉实验和免疫共沉淀技术;靶基因与蛋白质互作的方法主要有:酵母单杂交系统、染色质免疫沉淀技术、凝胶电泳迁移技术和荧光素酶报告系统。

Plant transcription factors regulate the expression of vast downstream genes and play an important role in the regulation of plant growth and development, as well as its response to environment. With the development of transcription factors in model plants and crops, the research methods of transcription factors are also being innovated. We describe the research methods of plant transcription factors from two aspects: protein-protein interaction and target gene-protein interaction, so as to provide a reference method for the related research of plant transcription factors. Protein-protein interaction methods include: yeast two-hybrid system, fluorescence resonance energy transfer, bimolecular fluorescence complementation, pull down and co-immunoprecipitation;methods for target gene-protein interaction include: yeast one-hybrid system, chromatin immunoprecipitation, electrophoretic mobility shift assay and luciferase reporter assay.