摘要
通过对川贝母外植体愈伤组织的培养,建立了培养周期短、愈伤组织生长效率高的培养体系。利用多因子正交实验设计及分析,筛选出最佳愈伤组织诱导培养基是MS + 2.0 mg/L 6-BA+ 0.5 mg/L 2,4-D + 0.1 mg/L NAA。通过分析原药材与愈伤组织中总生物碱的含量,从而考察愈伤组织生物量及药效物质积累的影响,并揭示正常培养的愈伤组织中总生物碱含量稍低于原药材。因此,通过培养愈伤组织是一种获得大量川贝母活性物质的有效方法,为川贝母产品生产的提供一种原材料途径。
A highly effective and rapid culture system of callus was developed by cultivating callus from ex-plants of Fritillaria cirrhosa. The design and analysis of multifactorial orthogonal experiments showed the optimum medium for callus induction was MS + 2.0 mg/L 6-BA+ 0.5 mg/L 2,4-D + 0.1 mg/L NAA. By analyzing the content of total alkaloids in the original medicinal materials and cal-lus, the effects of biomass and accumulation of effective substances in the callus were investigated, and it was revealed that the content of total alkaloids in the normal callus was slightly lower than that in the original medicinal materials. Therefore, callus culture is an effective way to obtain a large number of active substances of Fritillaria cirrhosa, which provides a way to obtain raw materials for the production of Fritillaria cirrhosa.
出处
《植物学研究》
2020年第1期59-64,共6页
Botanical Research
基金
四川省科技计划项目(2018NZ0091)、四川省级公益性科研院所基本科研业务费项目、农业农村部杂粮加工重点实验室开放基金资助(No.2018CC12)、四川省中医药管理局科研项目(NO.2016ZY008)共同资助。
