月见草根多糖提取工艺优化及其抗氧化活性研究

Optimization of Extraction Process and Antioxidant Activity in Vitro for Polysaccharide from Oenothera erythrosepala Root Polysaccharide

为了更好的开发月见草根中的多糖,本文运用单因素和响应面法对月见草根中多糖的最佳提取工艺进行了一系列研究。根据最佳提取工艺,对不同时期和不同土质中月见草根多糖的动态积累规律进行了的分析,并研究了月见草根中多糖对过氧化氢氧化损伤的RAW264.7细胞的修复作用。结果显示,用微波提取法提取月见草根中多糖比用超声提取法和传统工艺法有更好的提取效果。通过单因素和响应面法分析,在微波提取法中,月见草根中多糖的最佳提取条件:微波功率460 w、浸提时间25 min、料液比1:20,在此条件下的提取率为78.55%。在最佳提取方法下,对不同时期和不同土质中月见草根多糖的动态积累规律研究得知,11月份的月见草根多糖的含量最高为75.61%,而砂质中种植的月见草根的多糖含量高于壤土。抗氧化实验显示月见草多糖对过氧化氢氧化损伤的RAW264.7是有明显的修复效果。

To better explore the polysaccharides of Oenothera erythrosepala, the optimal extraction process of Oenothera erythrosepala polysaccharides was studied with the response surface method under a single factor. The content of polysaccharides from Oenothera erythrosepala in different periods was determined. Meanwhile, the repairing effect of polysaccharides from Oenothera erythrosepala was evaluated using RAW264.7 cells damaged by hydrogen peroxide. The results showed that the microwave extraction is more effective than the ultrasonic extraction and the traditional extraction. Through the single factor and the response surface analysis, the best extraction conditions of polysaccharide were microwave power 460 w, extraction time 25 min, feed liquid ratio 1:20, and the extraction rate under this condition was 78.55%. The contents of Oenothera erythrosepala po-lysaccharide in the different periods and soil showed that the highest content of polysaccharide was in November and sand. The activity test showed that Oenothera erythrosepala polysaccharide can effectively repair the damage of RAW264.7 by hydrogen peroxide oxidation.