黄花蒿种子超低温保存方法初探

Preliminary Study on Cryopreservation of Artemisia annua L. Seeds

试验采用直接冷冻法、缓慢冷冻法、玻璃化法对黄花蒿种子进行超低温保存初步研究,以保存前后种子在15℃、20℃、25℃时的发芽率来检测超低温保存效果。结果表明经过液氮冻存72 h后,采用缓慢冷冻法保存后的种子发芽率高,平均在84%以上,且超低温保存前后黄花蒿种子都在20℃时发芽率最高,分别达到94%和91.5%。研究表明黄花蒿种子超低温保存宜采用缓慢冷冻法,保存后恢复培养时的适宜发芽温度为20℃。

To study the cryopreservation method of Artemisia annua L. seeds. Direct freezing, slow freezing and vitrification methods were used to cryopreserve the seeds of A. annua. The germination rates at 15˚C, 20˚C and 25˚C before and after cryopreservation were used to evaluate the cryopreserved efficiency. After cryopreservation, slow freezing method had higher germination rates than direct freezing method and vitrification method. And before and after cryopreservation, the seed germination rates reached 94% and 91.5% respectively at 20˚C. The optimum cryopreservation method for seeds of A. annua was slow freezing, and the optimum germination temperature was 20˚C when cultivated after cryopreservation.