基于毛兰素含量为指标的石斛属植物品质鉴别分析

Quality Identification Analysis of Dendrobium Based on the Content of Erianin

目的:《中国药典》2015年版中将毛兰素含量定为鼓槌石斛品质的判断指标,论文采用高效液相色谱法测量39种石斛属中具有抗癌作用的毛兰素的含量。方法:采用Shim-pack GIS C18色谱柱(4.6 ×250 mm, 5 μm),以乙腈–三氟乙酸水溶液(pH = 3.25)为流动相,流速1.5 ml/min (水相0.9 ml/min,有机相0.6 ml/min),检测波长232 nm,柱温25℃,进样量20 μl。结论:39种石斛仅在鼓槌石斛中检测到毛兰素,含量为0.047%;本检测方法准确,快速,重复性好,适合石斛属植物毛兰素的含量鉴别分析。

Objective: In the 2015 edition of Chinese Pharmacopoeia, the content of erianin was specified as a judgment indicator of the quality of Dendrobium chrysotoxum. This paper determinates the content of erianin which has anticancer effect in 39 species of Dendrobium by high performance liquid chromatography (HPLC). Methods: The samples were separated on Shim-pack GIS C18 column (4.6 * 250 mm;5 μm) using a mobile phase consisting of trifluoroacetic acid-acetonitrile (pH = 3.25) at a flow rate of 1.5 mL/min (aqueous phase: 0.9 ml/min, organic phase: 0.6 ml/min) and detected at 232 nm, and the column temperature was kept at 25˚C. The injection volume was 20 μL. Conclusion: Among the 17 species of Dendrobium determined, erianin was detected only in Dendrobium chrysotoxum and the content was 0.047%. This method is accurate, rapid and suitable for the identification and analysis of the content of erianin in Dendrobium.