荆州市花生白绢病菌的分离鉴定

Isolation and Identification of Pathogen of Peanut Southern Blight in Jingzhou City

为明确引起湖北省荆州市花生白绢病的病原菌种类,本试验采用组织分离法、形态学鉴定法和基于ITS基因序列的分子鉴定法,对采自荆州市具有典型白绢病症状的花生植株进行病原菌的分离、纯化和鉴定。结果表明,引起花生白绢病的病原菌(QZ)被鉴定为罗氏阿太菌(Athelia rolfsii),属于担子菌亚门真菌;其无性态为齐整小核菌(Sclerotium rolfsii Sacc.),属半知菌亚门真菌。

To identify the pathogenic fungus that caused peanut southern blight in Jingzhou City of Hubei Province, the pathogens of peanut southern blight were isolated, purified and identified using the methods of tissue isolation, morphological identification, and molecular identification, which is based on ITS. The pathogenic fungi (QZ) that caused peanut southern blight were identified as Athelia rolfsii, which belonged to the subphylum basidiomycete fungus. The unnatural state is Sclerotium rolfsii Sacc., which belongs to the subphylum hemiknophyllum.