优质稻金稻919成熟胚再生体系的建立

Establishment of Mature Embryo Regeneration System of Good Quality Rice Variety Jindao919

以优质食味水稻品种金稻919成熟胚为外植体,设计4种激素配比诱导培养基和6种激素配比分化培养基,筛选最佳方案建立金稻919成熟胚再生体系。实验结果表明,Y2激素组合(4 mg/L 2,4-D)的诱导愈伤组织效果最佳,诱导率为77.3%;F5激素组合(4 mg/L 6-BA + 1 mg/L NAA)的分化成苗效果最佳,分化率为85.0%,成苗率为68.3%。本研究建立了金稻919成熟胚高效转基因再生体系,为利用基因工程技术定向改良奠定基础。

The author used the mature embryo of good quality rice variety Jindao919 as explants, and established the regeneration system of Jindao919 mature embryo through screening four kinds of hormone combination induction medium and six kinds of hormone combination differentiation medium. The results showed that Y2 hormone combination (4 mg/L 2,4-D) had the best callus induction effect, with the highest calli induction rate of 77.3%;and F5 hormone combination (4 mg/L 6-BA + 1 mg/L NAA) had the best calli differentiation effect with the highest differentiation rate of 85.0% and the seedling formation rate of 68.3%. In this study, an efficient regeneration system of Jindao919 mature embryo was established, which laid a foundation for directional improvement by genetic engineering technology.