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基因组定向编辑技术——CRISPR/Cas9的研究进展

Advances in Genome Directional Editing Technologies of CRISPR/Cas9
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摘要 CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats/CRISPR-associated)是广泛存在于细菌及古生菌中的,由细菌体长期进化而形成的,能够降解入侵病毒或噬菌体DNA的适应性免疫系统。由于CRISPR/Cas的Ⅱ型系统比较简单,因此成为目前应用最为广泛的方法。本文主要介绍了CRISPR/Cas系统的基本结构、作用原理、技术特点、研究进展以及该技术可能的应用前景等方面内容。 CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated) refers to an adaptive immune system that is gained from the long-term evolution of the organism which is widespread in bacteria and archaea. The system is able to degrade the invading virus or phage DNA. TypeⅡ of CRISPR/Cas system has become the most popular method due to its simplicity. This paper summarizes the basic structure, principle, technical characteristics and the progress of CRISPR/Cas, as well as the application prospect of the technology.
作者 姚瑶 钱雪艳 郭东全 Yao Yao;Xueyan Qian;Dongquan Guo(Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun)
出处 《农业科学》 2014年第6期142-150,共9页 Hans Journal of Agricultural Sciences
关键词 CRISPR/Cas系统 Cas9蛋白 基因定点修饰 CRISPR/Cas System Cas9 Protein Targeted Genome Modification
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  • 1邓智年,魏源文,吕维莉,李杨瑞.MAR序列介导野苋菜凝集素基因在白菜中的表达[J].园艺学报,2007,34(2):381-386. 被引量:9
  • 2Marraffini LA, Sontheimer EJ. CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA. Science, 2008, 322(5909): 1843-1845.
  • 3Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet, 2010, 11(3): 181-190.
  • 4Mojica FJM, D I ez-Villasefior C, Garc ~ a-Mart f nez J, A1- mendros C. Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology, 2009, 155(Pt 3): 733-740.
  • 5Brouns SJJ, Jore MM, Lundgren M, Westra ER, Slijkhuis RJ, Snijders APL, Dickman MJ, Makarova KS, Koonin EV, van der Oost J. Small CRISPR RNAs guide antiviral defense in orokarvotes. Science, 2008, 321(5891): 960-964.
  • 6Hale CR, Zhao P, Olson S, Duff MO, Graveley BR, Wells L, Terns RM, Terns MP. RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell, 2009, 139(5): 945-956.
  • 7Marraffini LA, Sontheimer EJ. Self versus non-self discrimi- nation during CRISPR RNA-directed immunity. Nature, 463(7280): 568-571.
  • 8Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea. Science, 2010, 327(5962): 167-170.
  • 9Heidelberg JF, Nelson WC, Schoenfeld T, Bhaya D. Germ warfare in a microbial mat community: CRISPRs provide in- sights into the co-evolution of host and viral genomes. PLoS One, 2009, 4(1): e4169.
  • 10Wilmes P, Simmons SL, Denef VJ, Banfield JF. The dynamic genetic repertoire of microbial communities. FEMS Microbiol Rev, 2009, 33(1): 109-132.

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