液相色谱法测定黄瓜和土壤中精甲霜灵的残留

Determination of Mefenoxam Residue in Cucumber and Soil by LC

本文对精甲霜灵(Metalaxyl-M)在黄瓜和土壤上采用乙腈提取、Amino萃取小柱净化、LC-紫外进行检测。试验结果表明:黄瓜上添加标准品0.020、0.50、1.0 μg/g,回收率分别为84.20%、81.02%、87.73%;土壤中添加标准品0.02、0.5、1.0 μg/g,回收率分别为71.50%、92.86%、90.82%。通过田间试验样品检测得到的精甲霜灵的消解动态方程,其在黄瓜中半衰期为11.5 d,在土壤中半衰期为13.9 d。精甲霜灵在样品中最低检测浓度为0.02 mg/kg,最小检出量为4 ×10?11 g。

This work examined metalaxyl-M residue in cucumber and soil using acetonitrile extraction, amino extraction column purification, and LC-UV detection. The results showed that recovery of pesticide spiked in cucumbers, at 0.02, 0.5 and 1 μg/g, was 84.20%, 81.02%, and 87.73%, respectively;and in soil, when spiked at 0.02, 0.5 and 1 μg/g, recovery was 71.50%, 92.86%, and 90.82%, re-spectively. Digestion dynamic equation of metalaxyl-M was obtained by field test sample detection, of which the half-life period was 11.5 d in cucumber and 13.9 d in soil. The minimum determination concentration was 0.02 mg/kg in this sample, and the detectable limit of metalaxyl-M was es-timated to be 4 ×10?11 g.