西藏冬青稞种质资源的SSR标记遗传多样性分析

Analysis of Genetic Diversity with SSR Markers on Winter Barley Germplasm Resources in Tibet

为了明确西藏冬青稞种质资源的遗传多样性,并为冬青稞育种提供依据,利用分布于青稞7条染色体上的32对SSR引物对来自西藏的94份冬青稞材料的遗传多样性进行了分析。结果表明,32对引物共检测出115条多态性条带,平均每对引物扩增出3.6条多态性带,表明西藏冬青稞种质资源遗传基础较广,蕴藏着多种不同的等位基因,具有丰富的遗传多样性。聚类分析将材料分为六组,材料的来源于所研究的SSR引物位点相关性状具有独立性,基于SSR多态性的聚类与材料来源地区无相关性。

In order to clarify the genetic diversity of the Tibetan winter barley germplasm resources, and provide bases for winter barley breeding, genetic diversity of 94 winter barley samples collected from Tibet were analyzed, based on the 32 pairs of SSR primers distributed in 7 chromosomes of barley. The results showed that, 115 polymorphic bands were detected from 32 pairs of primers totally, and in an average, every pair of primers had been amplified to be 3.6 polymorphic bands, which indicated that, the genetic basis of Tibet winter barley germplasm was wide, and multi different alleles existed, who owned a wealth of genetic diversities. Cluster analysis divided materials into six groups, and the sources of materials were independent to the related traits of SSR primer loci for study. There were no correlations between the source regions of materials and the clusters based on SSR polymorphism.