魔芋PDS基因VIGS载体的构建及其功能鉴定

Construction and Functional Identification of VIGS Vector of PDS Gene from Amorphophallus konjac

本文采用PCR技术从魔芋中克隆了番茄红素脱氢酶(PDS)基因部分序列,该片段长度450 bp,分析表明该基因片段与烟草中PDS序列同源性为77%。经过双酶切构建了p TRV-PDS的VIGS载体,转入农杆菌浸染魔芋,感染的组培苗魔芋植株叶片出现白化表型,定量PCR结果表明魔芋PDS基因的mRNA丰度出现下调且下调了10%。

Phtyoene desaturase (PDS) gene fragment is cloned from Amorphophallus konjac into tobacco tattle virus vector (TRV) to obtain p TRV-PDS. The construction is transformed into Agrobacterium CV3101 and then infected N. benthamiana. Silencing of the PDS gene in N. benthamiana can result in the white leaf due to photo-bleaching, indicating successful establishment of the VIGS system. The albino phenotype of plant leaves appeared, and the expression of PDS gene in konjak was down regulated and the gene expression was down regulated by 10%.