摘要
本文采用PCR技术从魔芋中克隆了番茄红素脱氢酶(PDS)基因部分序列,该片段长度450 bp,分析表明该基因片段与烟草中PDS序列同源性为77%。经过双酶切构建了p TRV-PDS的VIGS载体,转入农杆菌浸染魔芋,感染的组培苗魔芋植株叶片出现白化表型,定量PCR结果表明魔芋PDS基因的mRNA丰度出现下调且下调了10%。
Phtyoene desaturase (PDS) gene fragment is cloned from Amorphophallus konjac into tobacco tattle virus vector (TRV) to obtain p TRV-PDS. The construction is transformed into Agrobacterium CV3101 and then infected N. benthamiana. Silencing of the PDS gene in N. benthamiana can result in the white leaf due to photo-bleaching, indicating successful establishment of the VIGS system. The albino phenotype of plant leaves appeared, and the expression of PDS gene in konjak was down regulated and the gene expression was down regulated by 10%.
作者
王自布
黄燕芬
曹剑峰
Zibu Wang;Yanfen Huang;Jianfeng Cao(Guizhou Normal College, Guiyang Guizhou;Institute of Medicinal Plant Biotechnology, Guizhou Normal College, Guiyang Guizhou)
出处
《农业科学》
2018年第4期384-389,共6页
Hans Journal of Agricultural Sciences
基金
贵州省科学技术厅项目:黔科合J字[2013]2246号
黔高教发[2017]158号:贵州省2017年一流大学重点建设项目师资团队建设子项目
贵州师范学院自然科学研究基金(2015BS005)。