大花独蒜兰无菌萌发及幼苗栽培基质研究

Study on Asepticgermination and Seedling Cultivation Substrate of <i>Pleione grandiflora</i>

对大花独蒜兰种子无菌萌发进行了研究,建立了一套大花独蒜兰组织培养的快速繁殖体系,并初步对大花独蒜兰幼苗期的栽培基质进行了筛选。以大花独蒜兰的种子为外植体,研究了6-BA和2,4-D两种激素不同质量浓度配比对大花独蒜兰小苗形成和生长的影响,并将发育较好的原球茎分别栽培于水苔、水苔和木屑、木屑与泥炭土的组合上,以确定较为适宜大花独蒜兰小苗生长的基质。结果表明:大花独蒜兰原球茎萌发生长最适培养基为1/2 MS + 1.0 mg/L 2,4-D + 1.0 mg/L 6-BA, PH5.8。幼苗生长较适宜的栽培基质为带透明盖的育苗箱 + 2~4 mm木屑:泥炭土(1:1)。

The aseptic germination of Pleione grandiflora seeds was studied, a set of rapid propagation system for tissue culture was established, and the culture medium at seedling stage was screened. Using the seeds of Pleione grandiflora as explants, the effects of different concentrations of 6-BA and 2,4-D on the formation and growth of seedlings were studied, and the well-developed protocorms were cultivated on water moss and the combination of water moss and sawdust, the combination of sawdust and peat soil respectively to determine the suitable substrate for the growth of seedlings. The results showed that the optimum medium for protocorm germination and growth was 1/2 MS+ 1.0 mg/L 2,4-D + 1. 0 mg/L 6-BA, PH 5.8. The suitable substrate for seedling growth was nursery box with transparent cover + 2~4 mm sawdust: peat soil (1:1).