油茶真假花粉形态及发育过程观察

Observation on the Morphology and Development of Normal and Pseudopollen of <i>Camellia oleifera</i>

油茶真假花粉在形态和发育过程上都存在差异。本研究通过观察花粉悬液、石蜡切片和开裂的新鲜花药,分析了油茶真假花粉的形态和发育过程的差异。结果表明,油茶真花粉为黄色三角形的可产生花粉管的细胞,而假花粉为白色半透明的外壁具有肋骨状纹饰的细胞;真花粉由小孢子母细胞发育而来,假花粉由药隔的薄壁细胞发育而来,并且蓝光波段下的花药自发荧光可以指示真假花粉的发育过程;假花粉成熟后彼此分离,花药开裂释放真花粉,花药完全开裂后真花粉集中分布在由花药边缘的假花分形成的圈内。

There are differences in morphology and development between normal and pseudopollen of C. oleifera. In this study, the differences in morphology and development of normal and pseudopollen of C. oleifera were analyzed by observing pollen suspension, paraffin sections and cracked anthers. The results show that the normal pollen of C. oleifera is a yellow triangular cell capable of producing pollen tube, while the pseudopollen is a white translucent cell with rib-like ornamentation, and the normal pollen develops from microspore mother cells. The pseudopollen develops from the parenchyma cells of the connective, and the anther autofluorescence under the blue light band can indicate the development of normal and pseudopollen. The pseudopollens separate from each other after maturity, then the anthers crack and release normal pollens. After complete dehiscence of anthers, normal pollens are concentrated in the circle formed by pseudopollen on the edge of the anther.