章姬草莓茎尖离体快繁研究

Study of in Vitro Rapid Propagation of Shoot Tips of Zhangji Strawberry

以“章姬”草莓为试材,以匍匐茎茎尖为外植体,研究了不同激素配比对茎尖增殖培养、生根培养的影响,以筛选出章姬草莓茎尖作为外植体最适的培养基。结果表明:茎尖接种在培养基上,最适的初代培养基为:MS + BA 0.5 mg/L,最适的增殖培养基为MS + NAA 0.1 mg/L + BA 0.5 mg/L,最适的生根培养基为MS + NAA 0.5 mg/L。

Taking “Zhangji” strawberry as test material and stolon tip as explant, the effects of different hormone ratio on shoot tip proliferation culture and rooting culture were studied, so as to select the stem tip of Zhangji strawberry as the most suitable culture medium. The results showed that the optimum primary culture medium was MS + BA 0.5 mg/L, the optimum proliferation medium was MS + NAA 0.1 mg/L + BA 0.5 mg/L, and the optimum rooting culture was MS + NAA 0.5 mg/L.