bFGF-Chitosan通过招募骨髓间充质干细胞加速其向神经细胞分化进程

bFGF-Chitosan Accelerates Its Differentiation into Nerve Cells by Recruiting Bone Marrow Mesenchymal Stem Cells

目的:探讨bFGF-chitosan将MSC诱导为神经细胞及其过程。方法:成年大鼠骨髓间充质干细胞分别与bFGF-chitosan和单纯可溶性bFGF共培养,利用nestin,Tuj-1,MAP2等指标分析其分化行为。并利用钙成像技术鉴定其具有神经细胞功能。结果:在诱导19天时在bFGF-chitosan组中能够发现大量神经球样结构,在单纯可溶性bFGF和条件培养基组中能够观察到少量神经球样结构。继续培养能够在bFGF-chitosan组中观察到大量Tuj-1阳性细胞存在并聚集在bFGF-chitosan附近。培养40天时能够观察到MAP2阳性细胞,并能够观察到钙瞬变现象。结论:bFGF-chitosan能够通过将MSC诱导为神经球样结构诱导为具有功能的神经细胞。

Objective: To investigate theinduction of MSC into nerve cells and its process by bFGF-chitosan. Methods: Adultrat bone marrow mesenchymal stem cells were cultured with bFGF-chitosan andsoluble bFGF respectively, and their differentiation behavior was analyzed by meansof nestin, Tuj-1 and MAP2. Calcium imaging technique was used to identify itsnerve cell function. Results: At 19 days of induction, a large number ofneurospheres could be found in the bFGF-chitosan group, and a small amount ofneurospheres could be observed in the single soluble bFGF and conditionalculture group. Continued culture was able to observe a large number of Tuj-1positive cells in the bFGF-chitosan group and concentrate near bFGF-chitosan.MAP2 positive cells can be observed at 40 days of culture and calcium transientphenomena can be observed. Conclusion: bFGF-chitosan can induce MSC as afunctional nerve cell by inducing it as a neurosphere structure.