期刊文献+

牛防御素BNBD4成熟肽的真核表达及纯化 被引量:1

Eukaryotic Expression and Purification of Mature Bovine Neutrophil β-Defensins 4
下载PDF
导出
摘要 牛β防御素4 (BNBD4)是一种天然存在于牛肺脏肺泡巨噬细胞内的抗菌肽,本研究拟通过克隆BNBD4成熟肽片段的编码区序列,连接至真核表达载体pPIC9K构建重组质粒mBNBD4-pPIC9K,转化至毕赤酵母菌株GS115诱导表达,镍柱纯化后,经Tricine-SDS-PAGE检测和Western Blot鉴定,成功获取重组蛋白mBNBD4,为进一步研究牛防御素BNBD4的作用机制提供试验材料。 Bovine neutrophil β-defensins 4 (BNBD4) is one of the antimicrobial peptides which naturally exists in bovine pulmonary alveolar macrophages. In this study we will clone the CDs sequence of mature BNBD4 gene, and connect it to the eukaryotic expression vector pPIC9K to construct recombinant plasmid mBNBD4-pPIC9K, and purify it by Ni-sepharose after inducing expression. Finally, we can successfully obtain the recombinant protein mBNBD4 by Tricine SDS-PAGE detection and Western Blot identify, therefore to provide the experimental materials for the further study of mature BNBD4.
出处 《生物医学》 CAS 2019年第4期169-176,共8页 Hans Journal of Biomedicine
基金 河南省高等学校重点科研项目计划(18A230005) 河南牧业经济学院学术带头人(14021321)。
  • 引文网络
  • 相关文献

参考文献1

二级参考文献9

  • 1Lamberty M,Zachary D, Lanot R, et al. Constitutive expression of a cysteine-rich antifungal and linear antibacterial peptide in a termite insect[J]. J Biol Chem, 2001,276(6) :4085-4092.
  • 2Jones R N,Pfaller M A. Bacterial resistance: a world-wide problem[J]. Diagn Microbiol Infect Dis, 1998,31 (2) :379-388.
  • 3Andress R,Koczulla R. Antimicrobial peptides: current status and therapeutic potential[J]. Drugs, 2003, 63(4) :389-406.
  • 4Jacob L, Zasloff M. Potential therapeutic applications of magainins and other antimicrobial agents of animal origin[J]. Ciba Found Symp, 1994,186 : 197- 216.
  • 5Stephane C, Max G, Francois B, et al. Purification and characterization of a scorpion defensin,a 4kDa antibacterial peptide presenting structural with insect defensins and scorpion toxins[J]. Biochem Biophys Res Commun, 1993,194(1):17-22.
  • 6Mingfu Niu,Xiang Li,Jianchao Wei,et al. The molecular design of a recombinant antimicrobial peptide CP and its in vitro activity[J]. Protein Expression and Purification, 2008,57 (1) : 95-100.
  • 7Sambrook J, Fritsch E F, Maniatis T. Molecular cloning: A laboratory manual[M]. 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1998.
  • 8Piers K L,Brown M H, Hancock R W. Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacterial[J]. Gene, 1993,134:7-13.
  • 9Bathurst I C, Brennan S O, Carrell R W, et al. Yeast KEX2 protease has the properties of a human proalbumin converting enzyme[J]. Science, 1987,1235 (4786): J 348--350.

共引文献7

同被引文献20

引证文献1

二级引证文献1

;
使用帮助 返回顶部