LCMS检测维生素的室内质控血清制备及可行性研究

Preparation and Feasibility Study of Internal Quality Control Serum for Vitamins Detection in LCMS

目的:研制稳定的维生素质控血清,用于实验室内部维生素检测的质量控制。方法:准确称取一定量的维生素A、25-羟基维生素D2、25-羟基维生素D3、维生素E和维生素K1溶于适量的二甲基亚砜中成为母液,适量添加至基质血清中,形成高、低质控血清。分装成1 ml/支,分别贮存在4℃、−20℃和−80℃环境中,以观察其在不同贮存条件下的稳定性和均一性。结果:4℃保存的质控血清,从第4天起维生素K1值迅速下降,其他维生素(A、D2、D3和E)在一周后检测值也明显下降,−20℃和−80℃保存的质控血清中几种维生素的检测值都均可稳定1年以上;均一性检测表明样品间无显著性差异(F值 0.05),均一性良好。结论:本研究制备的质控血清具有良好的均一性和稳定性,可用于实验室内部维生素检测的质量控制。

Objective: To develop the stable vitamin quality control serum for the quality control of vitamin testing in the laboratory. Methods: A certain amount of vitamin A, 25-hydroxyvitamin D2, 25- hydroxyvitamin D3, vitamin E and vitamin K1 was dissolved in the appropriate dimethyl sulfoxide to form mother liquor and added to the matrix serum into high- and low-quality control serum. Repacked into 1 ml/bottle and stored in 4˚C, −20˚C, and −80˚C environments to observe their stability and uniformity under different storage conditions. Results: In 4˚C environments, concentration of vitamin K1 in the quality control serum was decreased rapidly from day 4, and other vitamins (A, D2, D3 and E) did so after a week. The detection values of several vitamins in the quality control serum in −20˚C and −80˚C were stable for more than 1 year;uniformity tests showed no significant difference between samples (F value 0.05), the results showed good uniformity. Conclusion: The quality control serum prepared in this study has good uniformity and stability, and can be used for the quality control of internal laboratory vitamin testing.