AgNPs@ZIF-8@HA的制备及其对两种耐药菌的体外抗菌活性

Preparation of AgNPs@ZIF-8@HA and Its Antibacterial Activities against Two Drug-Resistant Bacteria in Vitro

目的:合成基于金属–有机框架(ZIF-8)的复合纳米材料AgNPs@ZIF-8@HA,并研究其对耐甲氧西林金黄色葡萄球菌(MRSA)、耐万古霉素肠球菌(VRE)的体外抗菌活性。方法:采用“化学液相法”合成ZIF-8;以NaBH4为还原剂,原位还原AgNO3,将纳米银颗粒负载到ZIF-8的孔道中,表面包覆透明质酸构建AgNPs@ZIF-8@HA复合纳米材料,通过扫描电子显微镜(Scanning electron microscope, SEM)、透射电子显微镜(Transmission electron microscope, TEM)、X-射线粉末衍射(X-ray power diffraction, PXRD)、傅里叶红外光谱(Fourier transfer-infrared spectrometry, FTIR)进行结构和形貌表征。微量肉汤稀释法测定AgNPs@ZIF-8@HA对两种耐药菌的最低抑菌浓度,通过“平板涂布观察”研究AgNPs@ZIF-8@HA的体外抗菌活性。结果:AgNPs@ZIF-8@HA对MRSA的最低抑菌浓度(MIC)为80 μg/ml;AgNPs@ZIF-8@HA对VRE的最低抑菌浓度为20 μg/ml。结论:AgNPs@ZIF-8@HA是一种对MRSA和VRE敏感的抗菌纳米材料。

Purpose: Composite nanomaterial AgNPs@ZIF-8@HA based on metal-organic framework (ZIF-8) was synthesized and its in vitro antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) were studied. Methods: ZIF-8 was synthesized by chemical liquid phase method. Silver nanoparticles were loaded into the channels of ZIF-8 using AgNO3 reduced in situ by reducing agent NaBH4. Then, hyaluronic acid was coated to construct AgNPs@ZIF-8@HA composite nanomaterial. The structure and morphology were characterized by SEM, TEM, PXRD and FTIR. The minimum inhibitory concentration of AgNPs@ZIF-8@HA against two drug-resistant bacteria was determined by broth microdilution method, and the antibacterial activities of AgNPs@ZIF-8@HA in vitro were studied by “plate coating observation”. Results: The minimum inhibitory concentration (MIC) of AgNPs@ZIF-8@HA against MRSA was 80 μg/ml. The minimum inhibitory concentration of AgNPs@ZIF-8@HA against VRE was 20 μg/ml. Conclusion: AgNPs@ZIF-8@HA is a kind of antibacterial nanomaterial sensitive both to MRSA and VRE.