罗伊氏乳酸杆菌(Lactobacillus reuteri) GKD2改善高脂高糖饮食动物模式之血糖调控

Lactobacillus reuteri GKD2 Improves Blood Glucose Regulation in High-Fat and High-Sugar Diet Animal Model

葡萄糖耐受不良(impaired glucose tolerance)系指生理对血糖调控能力较差的状态,虽无明显临床症状,却为糖尿病(diabetes)或心血管疾病(cardiovascular diseases)的病变前兆。此阶段往往被忽视,或医疗建议的饮食控制与运动对于此类族群的实施效果有限。益生菌(probiotics)系指对人体机能有益的细菌,可参与人体的生理代谢、肠道菌相调整等,广受市场接受,因此具应用于糖尿病病变前期对策的潜能。本实验以高脂、高糖(high fat-high sugar, HF-HS)的饮食方式诱导C57BL/6JNarl小鼠发生葡萄糖耐受不良的状况,模拟常见之葡萄糖耐受不良成因。HF-HS小鼠分别补充8周低剂量(20.5 mg/kg/day)与高剂量(102.5 mg/kg/day)的罗伊氏乳酸杆菌(Lactobacillus reuteri) GKD2菌粉。在口服葡萄糖耐受性试验(oral glucose toerance test)中,相比单纯HF-HS饮食组,摄入菌株GKD2的HF-HS小鼠,其餐后血糖值有较低的趋势。此外,血糖调节的相关指标瘦体素(leptin)在益生菌组观察到下降的趋势;非空腹的胰岛素(insulin)浓度以高剂量GKD2组呈显著下降(p p L. reuteri GKD2有助于高脂高糖饮食者的血糖调控与生理代谢。

Impaired glucose tolerance refers to a state of poor physiological ability to regulate blood sugar. Although there are no obvious clinical symptoms, it is a precursor to diabetes or cardiovascular diseases. This stage is often ignored, or medically recommended diet control and exercise have limited effect on this group of people. Probiotics refer to bacteria that are beneficial to human body functions. They can participate in the body’s physiological metabolism and balance the gut microbiota. They are widely accepted by the market and therefore have the potential to be used in early stage countermeasures for diabetes. In this experiment, a high-fat, high-sugar (HF-HS) diet was used to induce glucose intolerance in C57BL/6JNarl mice, simulating the common causes of glucose intolerance. HF-HS mice were supplemented with low-dose (20.5 mg/kg/day) and high-dose (102.5 mg/kg/day) Lactobacillus reuteri GKD2 bacterial powder respectively for 8 weeks. In the oral glucose tolerance test, compared with the simple HF-HS diet group, the postprandial blood glucose levels of HF-HS mice consuming strain GKD2 tended to be lower. In addition, leptin, a related marker of blood sugar regulation, showed a downward trend in the probiotic group;non-fasting insulin concentration showed a significant decrease in the high-dose GKD2 group (p p L. reuteri GKD2 contributes to blood sugar regulation and physiological metabolism in those high-fat and high-sugar diets.