Rh-bFGF重组大肠杆菌的发酵工艺优化

Optimisation of the Fermentation Process of Rh-bFGF Recombinant Escherichia coli

Rh-bFGF重组大肠杆菌发酵工艺优化,根据培养基的组成成分,以菌体生长密度为检测值对重组大肠杆菌发酵条件所需碳源、有机氮源、无机氮源、无机盐离子、pH值、接种量进行单因素法和响应面法优化,得到最适发酵条件为:酵母粉3% (W/V)、豆粕0.5% (W/V)、氯化钠0.5% (W/V)、尿素0.5% (W/V)、pH 6.2、接种量5% (V/V)。当装液量为100 mL时,菌体生长密度OD600能达到2.66,相较于LB (OD600 = 1.5)培养基提升约77%,大大缩短发酵时间,达到节约成本的目的。

Optimisation of fermentation process of rh-bFGF recombinant E. coli. rh-bFGF recombinant E. coli fermentation process was optimised by one-way and response surface methods based on the composition of the medium, and the carbon source, organic nitrogen source, inorganic nitrogen source, inorganic salt ions, pH, and the amount of inoculum required for the fermentation conditions of the recombinant E. coli, according to the composition of the medium, with the growth density of the bacterium as the assay, and the optimal fermentation conditions were obtained as follows: 3% yeast powder (W/V), 0.5% soybean meal (W/V), NaCl 0.5% (W/V), urea 0.5% (W/V), pH 6.2, and inoculum 5% (V/V). When the volume of liquid is 100 mL, the density of bacterial growth OD600 can reach 2.66, compared with LB (OD600 = 1.5) medium to improve about 77%, greatly shorten the fermentation time, to achieve the purpose of cost savings.