蚕丝纬编针织网的细胞相容性及体外降解性实验研究

Experimental Study on Cytocompatibility and in Vitro Degradability of Silk Weft Knitted Mesh

目的:蚕丝纬编针织网支架结合骨髓间充质干细胞(BMSCs)细胞片制成组织工程支架,并对其进行细胞相容性观察,以及蚕丝纬编针织网支架的体外降解性。方法:通过密度梯度离心法结合贴壁培养法提取兔BMSCs,通过维生素C培养法制备BMSCs细胞片。采用纬编针织法制备蚕丝网状支架,将BMSCs细胞片覆盖于该支架上体外培养2周,观察细胞在支架上的生长情况。并进行蚕丝纬编针织网支架为期1年的体外降解性的力学性能及质量变化测试。结果:提取的BMSCs生长旺盛。支架-BMSCs细胞片培养2周的扫描电镜观察显示:BMSCs黏附于支架呈立体生长,增殖良好。通过体外1年降解实验,蚕丝纬编针织网状支架降解速率非常缓慢,力学性能及质量变化很小。结论:蚕丝纬编针织网-BMSCs细胞片具有良好的细胞相容性,蚕丝纬编针织网体外降解速率非常缓慢,可以尝试作为组织工程韧带/肌腱的支架材料。Objective: Silk weft knitted mesh scaffolds were combined with bone marrow-derived mesenchymal stem cells (BMSCs) to form tissue engineering scaffolds, and the cytocompatibility and degradability of silk weft knitted mesh scaffolds were observed. Methods: BMSCs were extracted by density gradient centrifugation combined with adherent culture, and BMSCs cell sheets were prepared by vitamin C culture. A silk mesh scaffold was prepared by weft knitting method. BMSCs cell sheets were covered on the scaffold and cultured for 2 weeks in vitro to observe the growth of cells on the scaffold. The mechanical properties and quality changes of silk weft knitted mesh scaffolds were tested for 1 year. Results: The extracted BMSCs grew strongly. Scanning electron microscopy (SEM) observation of BMSCs cells cultured on scaffolds for 2 weeks showed that BMSCs adhered to scaffolds in stereoscopic growth and proliferation. After 1 year in vitro degradation experiment, the degradation rate of silk weft knitted mesh scaffold is very slow, and the mechanical properties and quality changes are very small. Conclusion: BMSCs cell sheet with silk weft knitted mesh has good cytocompatibility, and the degradation rate of silk weft knitted mesh is slow in vitro, which can be used as scaffolds for tissue engineering ligaments/tendons.