拟南芥光周期开花路径的建模与分析

Modeling and Analysis of Photoperiodic Flowering in Arabidopsis thaliana

植物开花机制是一个复杂而精密的调控网络。组学分析表明,几乎所有光周期开花途径的相关基因或蛋白的昼夜节律性表达都受到生物钟的调控,其中一些关键基因,CYCLING DOF FACTOR 1 (CDF1)、CONSTANS (CO)和FLOWERING LOCUS T (FT)等在光周期条件下发挥着重要作用。本研究根据拟南芥生物钟对开花相关基因的调控关系,建立光周期开花路径的微分方程模型。模拟结果表明,在长日照下开花相关基因保持着昼夜节律振荡,FT积累到一定阈值,植物才能开花。此外,基于模型残差的平稳性和纯随机性检验的修正模型,再现了FT的积累量的变化趋势,为预测开花时间提供了一个可靠的分析工具。

Plant flowering mechanism is a complex and sophisticatedregulatory network. Omics analysis showed that the circadian rhythmicexpression of almost all genes or proteins related to photoperiodic flowering pathway is regulated by the circadianclock, and some key genes, CYCLING DOF FACTOR 1 (CDF1), CONSTANS (CO) and FLOWERING LOCUS T (FT) play important rolesunder photoperiodic conditions. In this study, adifferential equationmodel for photoperiodic flowering pathway was developed based on the regulatory relationship of flowering genes in Arabidopsis thaliana. The simulation results showed that the flowering related genes maintained circadian oscillations under long-day. FT must accumulate to a certain threshold before plants could flower. In addition, the modified model based on the stationarity and pure randomness test of model residuals simulated the trend of FT accumulation, which provided a reliable analytical tool for predicting flowering time.