南沙参TLC鉴别和HPLC指纹图谱研究

Study on TLC Identification and HPLC Fingerprint of Adenophora radix

目的:对南沙参进行TLC (薄层色谱)鉴别并建立HPLC (高效液相)指纹图谱方法,为其质量控制提供专属性更好的测试方法。方法:TLC采用药典方法;HPLC色谱柱Thermo AcclaimTM 120 C18 (4.6 ×250 mm, 5 μm),流动相乙腈-0.5%磷酸水溶液,梯度洗脱,流速为1.0 mL/min,检测波长为203 nm,柱温30℃;采用中药色谱指纹图谱评价系统对不同批次样品进行相似度分析。结果:对9批药材进行了TLC鉴别和建立了9批药材HPLC指纹图谱,确定3个共有色谱峰,方法学验证结果符合指纹图谱相关要求。结论:该方法简单、可靠,可用于南沙参药材的鉴别和质量控制。

Objective:To identify Adenophora radix with TLC and to establish HPLC fingerprint of Adenophora radix to provide better specificity test method for the quality control.Methods:Pharmacopoeia TLC method.HPLC Thermo AcclaimTM 120 C18(4.6×250 mm,5μm)was used with acetonitrile-0.5%phosphoric acid water solution as mobile phase in a gradient mode at the flow rate of 1.0 mL/min.The detection wavelength was 203 nm and the temperature of column was set at 30?C.Similarity evaluation system for chromatographic fingerprint of TCM was applied to analyze different batches of Adenophora radix samples.Results:TLC identification was carried out on 9 batches of medicinal materials,and 9 batches of HPLC fingerprints were established.Three common chromatographic peaks were determined.The results of methodological verification met the related requirements of fingerprints.Conclusion:This method is simple and reliable,and can be used for the identification and quality control of Adenophora radix.