尼罗罗非鱼皮胶原蛋白乳酸提取工艺条件优化及理化特性研究

Collagen Extraction from Nile Tilapia (Oreochromis niloticus) Skin: Optimization of Extraction Conditions Using Response Surface Methodology and Physicochemical Characterization

以罗非鱼皮为原料,乳酸为介质提取胶原蛋白。通过单因素实验分别考察pH值、液固比和提取时间对胶原蛋白提取率的影响,在此基础上,利用Box-Behnken中心组合实验设计和响应面分析法对乳酸提取胶原蛋白条件进行优化,并对胶原蛋白的理化性质进行评估。结果表明:乳酸提取罗非鱼皮胶原蛋白(ASC)的优化工艺条件为pH2.1、液固比65:1 (mL/g)、时间44 h,在此条件下罗非鱼皮胶原蛋白提取率可达85.57%,ASC的亚基组成形式为(α1)2α2,热变性温度为32.56℃,呈现均匀的纤维结构。

This study was undertaken to optimize the acidic extraction of collagen from Nile tilapia (Oreoch-romis niloticus) skin. Lactic acid was used to extract collagen. Further, three process conditions affecting the yield of collagen extraction with lactic acid, such as pH value, liquid-to-solid ratio and extraction time, were optimized by single factor experiment. According to this, Box-Behnken ex-perimental design combined with response surface methodology was taken to optimize the ex-tracting conditions of collagen using lactic acid. Based on the optimized conditions, physicochemical properties of collagen were evaluated. The results indicated that the optimal conditions for collagen extraction were pH2.1, liquid-to-solid ratio 65:1 (mL/g) and extraction time 44 h, under which a yield of collagen of 85.57% was obtained. The ASC showed that SDS-PAGE pattern, the thermal denaturation temperature, and morphology were (α1)2α2, 32.56℃, and evenly fibrous structure, respectively.